Juan Carlos RENGIFO-GONZALEZ

Qualité : PhD Student

My academic background includes Molecular Parasitology, Biochemistry and Molecular and Cell Biology. During my Master studies I was working with bacterial membrane proteins in order to characterize potential targets for new antibiotic-based treatments by applying Protein Crystallography methods.

Joining the SABNP Laboratory as PhD student was a great opportunity to continue working in the Structural Biology field. In this sense, my thesis subject concerns the structural characterization of RNA-binding proteins (RBPs) involved during neurodegeneration. Particular features of those RNA-protein complexes are being analyzed at the atomic level by applying Nuclear Magnetic Resonance (NMR) on labeled biomolecules. I am also interested in analyzing the most prominent structural hallmarks of these biological complexes by in cellulo and in silico methods in order to better understand the mechanism by which RBPs bind to their nucleic acid targets and how structural changes could be involved in neurodegenerative diseases (Alzheimer, Parkinson, Amyotrophic Lateral Sclerosis).

 

2016 – Current: Third year of PhD in Life Sciences. University of Evry Val d’Essonne / University Paris-Saclay. France.

2014 – 2016: Master in Biochemistry and Molecular and Cell Biology. University of Liège. Belgium.

2013 – 2014: Preparatory Year for Master in Biochemistry and Molecular and Cell Biology. University of Namur. Belgium.

2006 – 2013: Bachelor in Biology. University of Los Andes. Venezuela.

COMPUTATIONAL SKILLS: Microsoft Office Package, Image J, SigmaPlot, PyMol, Discovery Studio, ApE, EMBOSS Explorer Tools, ExPASy Bioinformatic Tools, TopSpin, BMRB, CCPNMR Analysis, HADDOCK, GRAMM-X.

LABORATORY SKILLS:

– Bacterial and yeast cultures

– Molecular Biology (PCR, nucleic acids purification, site-directed mutagenesis, in vitro RNA synthesis).

– Protein purification methods (Cellular Lysis, Chromatography Techniques, Protein Visualization and Quantitation)

– Approach in SAXS, Protein Crystallography, and Computational Predictions on biomolecular complexes.

– Analysis of protein structures by RMN

– Protein labelling and deuteration

– Reversible and irreversible modification and bioconjugation of proteins.

LANGUAGES:                    

  • SPANISH: Mother Tongue
  • ENGLISH: Advanced
  • FRENCH: Advanced