Manel TARI

Qualité : PhD Student

Background

I started my university studies in Algiers where I completed a Bachelor and a Master’s degree in genetics from the Houari Boumediene University. Then, I moved to Nancy, France where I completed a Master’s degree in Cellular and molecular biology with an emphasis on Molecular and cellular aspects of physiopathology. During the two years I spent in Nancy, my passion grew for the research of the mechanisms related to RNA processing and maturation. Finally, I joined the SABNP Laboratory as a PhD student where I study the role of an RNA binding family in the regulation of mRNA maturation.

Most genes in higher eukaryotes are initially transcribed as pre-mRNAs from which intervening sequences are removed, yielding mature mRNAs, a process known as splicing. Different combinations of segments of the pre-mRNA can often be joined together during splicing. This is called alternative splicing. Genome wide analyses of transcripts have revealed that alternative splicing is prevalent in metazoans and has an essential contribution to the diversity of protein isoforms that are expressed from a limited number of genes. Furthermore alternative splicing is highly regulated and cell type specific. A nowadays challenge is to understand how alternative splicing is regulated and what are the respective functions of the different spliced isoforms.

These last years, several RNA-binding proteins (RBPs) have been identified. We hypothesize that these proteins are involved in major regulations of alternative splicing events.

In this context, the thesis work have the following aims:

1) To characterize the protein partners of these RBPs in an effort to address the possibility of a combinatorial role of these proteins in recognizing the splice sites.

2) To identify the alternative splicing events that depend on these RBPs, using knock down (RNA silencing) and transcriptome analyses. The identified targets are validated experimentally and common features of the targets are determined to infer and test the molecular mechanisms of these RBPs.

These studies bring important results to understand the molecular mechanisms of alternative splicing whose defects are widely responsible for cancers and developmental disorders.

October 2015 – October 2018

PhD: Doctoral school SDSV, « Study of the role of UHM domain proteins in the regulation of alternative splicing », University of Evry Val d’Essonne, Evry, France

September 2013 – June 2014

Master 2: Biosciences and health engineering, « Molecular and integrative aspects of physiopathology » University of Lorraine, Nancy, France

September 2012 – June 2014

Master 1: Health and life sciences, « Cell biology and physiopathological basis of therapeutics »University of Lorraine, Nancy, France

September 2006 – June 2011

Licence and Master 2: Cellular and molecular biology « Fundamental and applied genetics» University of sciences and technology Houari Boumediene, Algiers, Algeria

September 2006 Baccalauréat S

Abd El Moumen High school, Algiers, Algeria 

Training and internships

January 2014 – 30 June 2014

Master 2 internship: Study of the role of the survival of motoneurons complex (SMN), whose deficit leads to spinal muscular atrophy, in the U3 snoRNP biogenesis, that is essential to ribosomal RNAs maturation.

Supervised by Dr Séverine Massenet, IMoPA Laboratory, CNRS UMR7365 – University of Lorraine, Nancy, France

January 2013 – February 2013

Master 1 Internship: Study of the interactions between the components of the survival of motoneurons complex and the factors involved in C/D and H/ACA box ribonucleoparticles assembly.

Supervised by Dr Séverine Massenet, IMoPA Laboratory, CNRS UMR7365 – University of Lorraine, Nancy, France

January 2011 – June 2011

Master 2 Internship: Genetic aspect of cervical cancer

Supervised by Pr Nacer Abdelali and Dr Nabila Seridi Cell biology laboratory (CHU Parnet) – Genetics Laboratory (Pasteur institute, Algiers)

Skills

Molecular biology

RNA and DNA extraction; Reverse transcription; PCR and q-PCR; Fluidigm qPCR technology; Electrophoresis and western blot; Northern-blot; RNA transcription in vitro

Proteins extraction; immunoprecipitation; GST pull-down

Double hybride technics (yeast)

Cellular biology

Cell culture; Stable cell line constructions

Cell transfection; viral supernatant production; cell transduction; Immunostaning;

Proximity ligation assay;

Informatics

Adobe Illustrator, Adobe Photoshop

Serial Cloner, for sequences alignments, restriction sites prediction…

ImageJ,

Cell profiler, for immune staining analyses and quantification

Specific training

Initiation to scientific mediation

Languages

French: Maternal tongue

Arabic: Maternal tongue

English: University diploma of English in a professional situation, advanced level (C1)